Recombinant Human CD274 protein, C-Fc Tag

Product Description

Cat

IMP-049

Official Symbol

CD274

Product Overview

Human PD-L1, Fc Tag (HPLC-verified) (PD1-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 19-Arg 238 (Accession # NP_054862.1). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.3 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human PD-L1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-L1, Fc Tag was more than 90% and the molecular weight of this protein is around 115-150 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-1, Strep Tag at 2 ug/mL (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 4-63 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 uL/well) can bind Anti-PD-L1 MAb with a linear range of 0.2-2 ng/mL.

Bioactivity-ELISA 3

Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 0.01-0.313 ug/mL.

Bioactivity-ELISA 4

Serial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag: Biotinylated Human PD-1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2966 ug/mL.

Bioactivity-BLI 1

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L1, Fc Tag with an affinity constant of 38.9 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-L1, Fc Tag on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 18 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human PD-L1, Fc Tag on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin with an affinity constant of 4.8 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 4

Loaded Human PD-L1, Fc Tag on Protein A Biosensor, can bind Human Human PD-1, Strep Tag with an affinity constant of 2.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that Human PD-L1, Fc Tag can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human PD-L1, Fc Tag (HPLC-verified) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-L1 antibody. The concentration of PD-L1 used is 10 ug/mL. The IC50 is 12.92 ug/mL.

Bioactivity-Cell based assay 1

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag with an EC50 of 0.029 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry