Recombinant Human CD274 protein, C-Fc Tag

Product Description

Cat

IMP-049

Official Symbol

CD274

Product Overview

Human PD-L1, Fc Tag (HPLC-verified) (PD1-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 19-Arg 238 (Accession # NP_054862.1). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.3 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human PD-L1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-L1, Fc Tag was more than 90% and the molecular weight of this protein is around 115-150 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-1, Strep Tag at 2 ug/mL (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 4-63 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 uL/well) can bind Anti-PD-L1 MAb with a linear range of 0.2-2 ng/mL.

Bioactivity-ELISA 3

Immobilized Human B7-1, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 0.01-0.313 ug/mL.

Bioactivity-ELISA 4

Serial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag: Biotinylated Human PD-1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2966 ug/mL.

Bioactivity-BLI 1

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L1, Fc Tag with an affinity constant of 38.9 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-L1, Fc Tag on Protein A Biosensor, can bind Human B7-1, His Tag with an affinity constant of 18 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human PD-L1, Fc Tag on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin with an affinity constant of 4.8 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 4

Loaded Human PD-L1, Fc Tag on Protein A Biosensor, can bind Human Human PD-1, Strep Tag with an affinity constant of 2.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that Human PD-L1, Fc Tag can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human PD-L1, Fc Tag (HPLC-verified) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-L1 antibody. The concentration of PD-L1 used is 10 ug/mL. The IC50 is 12.92 ug/mL.

Bioactivity-Cell based assay 1

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag with an EC50 of 0.029 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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