Recombinant Human CD274 protein, C-hFc-Avi-His Tag, Biotinylated

Product Description

Cat

IMP-050

Official Symbol

CD274

Product Overview

Biotinylated Human PD-L1, Fc,Avitag,His Tag (PD1-H82F3) is expressed from human 293 cells (HEK293). It contains AA Phe 19-Arg 238 (Accession # NP_054862.1). This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi-His Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 54.0 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Protein length

Phe 19-Arg 238

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Biotinylated Human PD-L1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag with a linear range of 2-39 ng/mL.

Bioactivity-ELISA 2

Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag with a linear range of 0.02-0.625 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin (HPLC-verified): Biotinylated Human PD-L1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 ug/mL.

Bioactivity-FACS 1

Flow Cytometry assay shows that Biotinylated Human PD-L1, Fc,Avitag,His Tag can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Biotinylated Human PD-L1, Fc,Avitag,His Tag to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2 ug/mL. The IC50 is 0.64 ug/mL.

Bioactivity-Cell based assay 1

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag with an EC50 of 0.082 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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