Recombinant Human CD274 protein, C-hFc-Avi-His Tag, Biotinylated

Product Description

Cat

IMP-050

Official Symbol

CD274

Product Overview

Biotinylated Human PD-L1, Fc,Avitag,His Tag (PD1-H82F3) is expressed from human 293 cells (HEK293). It contains AA Phe 19-Arg 238 (Accession # NP_054862.1). This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi-His Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 54.0 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Protein length

Phe 19-Arg 238

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Biotinylated Human PD-L1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag with a linear range of 2-39 ng/mL.

Bioactivity-ELISA 2

Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag with a linear range of 0.02-0.625 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin (HPLC-verified): Biotinylated Human PD-L1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 ug/mL.

Bioactivity-FACS 1

Flow Cytometry assay shows that Biotinylated Human PD-L1, Fc,Avitag,His Tag can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Biotinylated Human PD-L1, Fc,Avitag,His Tag to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2 ug/mL. The IC50 is 0.64 ug/mL.

Bioactivity-Cell based assay 1

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag with an EC50 of 0.082 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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