Recombinant Human CD274 protein, C-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-630

Official Symbol

CD274

Product Overview

Biotinylated Recombinant Human CD274 protein(NP_001254635.1)(Phe 19-Arg 238), fused to C-His-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) with a linear range of 0.1-2 ng/mL.

Bioactivity-ELISA 2

Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Anti-PD-L1 MAb, Human IgG1 with a linear range of 0.2-3 ng/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate. can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.1-3 ng/mL.

Bioactivity-ELISA 4

Immobilized Monoclonal Anti-Human PD-L1 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) with a linear range of 0.1-2 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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