Recombinant Human CD274 protein, C-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-630

Official Symbol

CD274

Product Overview

Biotinylated Recombinant Human CD274 protein(NP_001254635.1)(Phe 19-Arg 238), fused to C-His-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) with a linear range of 0.1-2 ng/mL.

Bioactivity-ELISA 2

Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Anti-PD-L1 MAb, Human IgG1 with a linear range of 0.2-3 ng/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate. can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.1-3 ng/mL.

Bioactivity-ELISA 4

Immobilized Monoclonal Anti-Human PD-L1 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) with a linear range of 0.1-2 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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