Recombinant Human CD274 protein, C-Llama IgG2b Fc Tag

Product Description

Cat

IMP-631

Official Symbol

CD274

Product Overview

Recombinant Human CD274 protein(NP_001254635.1)(Phe 19-Arg 238), fused to C-Llama IgG2b Fc Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-Llama IgG2b Fc Tag

Form

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 53.0 kDa. The protein migrates as 65-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 0.01 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human PD-1, His Tag at 1 μg/mL (100 μL/well) can bind Human PD-L1, Llama IgG2b Fc Tag, low endotoxin with a linear range of 0.078-2.5 μg/mL.

Bioactivity-ELISA 2

Immobilized Human PD-L1, Llama IgG2b Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc Tag with a linear range of 20-313 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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