Recombinant Human CD274 protein, C-mFc Tag

Product Description

Cat

IMP-240

Official Symbol

CD274

Product Overview

Recombinant Human CD274 protein(AA Phe 19-Arg 238)(Accession # NP_054862.1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.6 kDa. As a result of glycosylation, the protein migrates as 65-85 kDa under reducing (R) condition, and 130-170 kDa under non-reducing (NR) condition (SDS-P

Protein length

Phe 19-Arg 238

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin was more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-1, Strep Tag at 1 ug/mL (100 uL/well) can bind Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin with a linear range of 5-78 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of Nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin: Human PD-1, Strep Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2391 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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