Recombinant Human CD274 protein, C-mFc Tag

Product Description

Cat

IMP-240

Official Symbol

CD274

Product Overview

Recombinant Human CD274 protein(AA Phe 19-Arg 238)(Accession # NP_054862.1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.6 kDa. As a result of glycosylation, the protein migrates as 65-85 kDa under reducing (R) condition, and 130-170 kDa under non-reducing (NR) condition (SDS-P

Protein length

Phe 19-Arg 238

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin was more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-1, Strep Tag at 1 ug/mL (100 uL/well) can bind Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin with a linear range of 5-78 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of Nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin: Human PD-1, Strep Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2391 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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