Recombinant Human CD274 protein, C-Strep Tag

Product Description

Cat

IMP-048

Official Symbol

CD274

Product Overview

Recombinant Human CD274 protein(AA Phe 19-Arg 238)(Accession # NP_054862.1) is expressed from human 293 cells (HEK293). This protein carries a twin strep tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Strep Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-L1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human PD-L1, Strep Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag with a linear range of 0.002-0.25 ug/mL.

Bioactivity-BLI 1

Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Human PD-L1, Strep Tag with an affinity constant of 0.891 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, Fc Tag, low endotoxin (HPLC-verified) on Protein A Biosensor, can bind Human PD-L1, Strep Tag with an affinity constant of 2.2 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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