Recombinant Human CD28 Protein (19-152aa), C-hIgG-His-Tagged

Product Description

Cat

IMP-9438

Official Symbol

CD28

Product Overview

Recombinant human CD28, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Description

CD28, also known as T-cell-specific surface glycoprotein CD28 isoform 1, is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. It ligation has also been shown to regulate Th1/Th2 differentiation.

Expression System

Baculovirus

Species

Human

Tag

C-hIgG-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Molecular Mass

42.4 kDa (376 aa)

Protein length

19-152

AA Sequence

<ADP>NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP<LEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH>

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

1 mg/mL (determined by absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Human CD80 is coated at 1 μg/mL (100 μL/well) can bind Human CD28. The ED50 range ≤ 10μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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