Recombinant Human CD300LB Protein (Ile55-His187), C-hFc-tagged

Product Description

Cat

IMP-10379

Official Symbol

CD300LB

Product Overview

Recombinant human CD300b/LMIR5 protein (Ile55-His187) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc

Predicted N Terminal

Ile55

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 42 kDa
SDS-PAGE: 46-59 kDa, reducing conditions

Protein length

Ile55-His187

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD300b/LMIR5 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human LMIR2/CD300C Fc Chimera binds with an ED50 of 1.5-9 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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