Recombinant Human CD300LB Protein (Ile55-His187), C-hFc-tagged
Product Description
Cat
IMP-10379
Official Symbol
CD300LB
Product Overview
Recombinant human CD300b/LMIR5 protein (Ile55-His187) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
Ile55
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 42 kDa
SDS-PAGE: 46-59 kDa, reducing conditions
Protein length
Ile55-His187
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD300b/LMIR5 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human LMIR2/CD300C Fc Chimera binds with an ED50 of 1.5-9 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD300b/LMIR5 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human LMIR2/CD300C Fc Chimera binds with an ED50 of 1.5-9 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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