Recombinant Human CD33 Protein (Asp18-His259), C-Fc-tagged, Atto 647N Conjugated
Product Description
Cat
IMP-10122
Official Symbol
CD33
Product Overview
Atto 647N conjugateg recombinant human Siglec-3/CD33 protein (Asp18-His259) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Disulfide-linked homodimer, labeled with Atto 647N via amines
Excitation wavelength: 647 nm
Emission wavelength: 667 nm
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-Fc
Predicted N Terminal
Asp18
Form
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Conjugate
Atto 647N
Molecular Mass
Predicted Molecular Mass: 53.4 kDa (monomer)
SDS-PAGE: 67-80 kDa, under reducing conditions
Protein length
Asp18-His259
Bio-activity
Measured by flow cytometry for its ability to bind anti-Human Siglec-3/CD33 Monoclonal Antibody conjugated fluorescent beads.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, labeled with Atto 647N via amines
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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