Recombinant Human CD33 Protein (Asp18-His259), C-Fc-tagged, Atto 647N Conjugated
Product Description
Cat
IMP-10122
Official Symbol
CD33
Product Overview
Atto 647N conjugateg recombinant human Siglec-3/CD33 protein (Asp18-His259) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Disulfide-linked homodimer, labeled with Atto 647N via amines
Excitation wavelength: 647 nm
Emission wavelength: 667 nm
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-Fc
Predicted N Terminal
Asp18
Form
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Conjugate
Atto 647N
Molecular Mass
Predicted Molecular Mass: 53.4 kDa (monomer)
SDS-PAGE: 67-80 kDa, under reducing conditions
Protein length
Asp18-His259
Bio-activity
Measured by flow cytometry for its ability to bind anti-Human Siglec-3/CD33 Monoclonal Antibody conjugated fluorescent beads.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, labeled with Atto 647N via amines
Storage
Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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