Recombinant Human CD33 Protein (Asp18-His259), C-hFc and 6×His-tagged
Product Description
Cat
IMP-7433
Official Symbol
CD33
Product Overview
Recombinant Human CD33 Protein with C-Human Fc (Glu99-Ala330) and 6×His was expressed in HEK293.
Description
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2, 3- and more avidly alpha-2, 6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Expression System
HEK293
Species
Human
Tag
C-hFc and 6×His
Form
Lyophilized from sterile PBS, pH 7.4. Normally 5-8 % trehalose is added as protectants before lyophilization.
Molecular Mass
53.8 kDa
Protein length
Asp18-His259
Purity
> 95%
Storage
Store the lyophilized protein at -20 centigrade. After reconstitution, store the protein at -80 centigrade for 12 months. Avoid repeated freezing and thawing.
Stability: 12 months from date of despatch.
Reconstitution
Reconstitute with deionized water.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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