IMP-7432

CD33

Purified recombinant protein of Human CD33 molecule (CD33), transcript variant 1 with C-hFc&His tag was expressed in HEK293.

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed: 10611343, PubMed: 15597323, PubMed: 11320212). Preferentially recognizes and binds alpha-2, 3- and more avidly alpha-2, 6-linked sialic acid-bearing glycans (PubMed: 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed: 28325905, PubMed: 10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed: 10556798, PubMed: 10206955, PubMed: 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed: 10206955, PubMed: 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed: 15597323).[UniProtKB/Swiss-Prot Function]

HEK293

Human

C-hFc&His

Lyophilized from a 0.2 um filtered solution of PBS, 5% Trehalose, 0.01%Tween 80, pH7.2

55.01 kDa

Asp18-His259

< 0.1 ng/μg

> 95% as determined by SDS-PAGE and Coomassie blue staining

Store at -80 centigrade.
Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.

lot specific