Recombinant Human CD33 Protein (Asp18-His259, Val257Leu), C-Fc-tagged, Alexa Fluor 488 Conjugated

Product Description

Cat

IMP-10119

Official Symbol

CD33

Product Overview

Alexa Fluor 488 conjugated recombinant human Siglec-3/CD33 protein (Asp18-His259) (Val257Leu) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm

Description

Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Asp18

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 488

Molecular Mass

Predicted Molecular Mass: 53.4 kDa (monomer)
SDS-PAGE: 67-85 kDa, under reducing conditions

Protein length

Asp18-His259, Val257Leu

Bio-activity

Measured by flow cytometry for its ability to bind anti-human Siglec-3/CD33 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human Siglec-3/CD33 Fc Chimera Alexa Fluor 488 that produces 50% of the binding response is 2.50-50.0 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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