Recombinant Human CD33 Protein (Asp18-His259, Val257Leu), C-Fc-tagged, Alexa Fluor 647 Conjugated

Product Description

Cat

IMP-10120

Official Symbol

CD33

Product Overview

Alexa Fluor 647 conjugated recombinant human Siglec-3/CD33 protein (Asp18-His259) (Val257Leu) with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Labeled with Alexa Fluor 647 via amines
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm

Description

Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-Fc

Predicted N Terminal

Asp18

Form

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Conjugate

Alexa Fluor 647

Molecular Mass

Predicted Molecular Mass: 53.4 kDa (monomer)
SDS-PAGE: 67-85 kDa, under reducing conditions

Protein length

Asp18-His259, Val257Leu

Bio-activity

Measured by flow cytometry for its ability to bind anti-human Siglec-3/CD33 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human Siglec-3/CD33 Fc Chimera Alexa Fluor 647 that produces 50% of the binding response is 0.50-20.0 ng/mL.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, -20 to -70 centigrade under sterile conditions after opening.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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