Recombinant Human CD33 Protein (Asp18-His259, Val257Leu), C-hFc-tagged
Product Description
Cat
IMP-10375
Official Symbol
CD33
Product Overview
Recombinant human Siglec-3/CD33 protein (Asp18-His259, Val257Leu) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
Asp18
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 53.4 kDa (monomer)
SDS-PAGE: 67-85 kDa, reducing conditions
Protein length
Asp18-His259, Val257Leu
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 1-4 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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