Recombinant Human CD33 Protein (Met17-His259), C-6×His and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10602

Official Symbol

CD33

Product Overview

Biotinylated recombinant human Siglec-3/CD33 protein (Met17-His259) with a 6-His-Tag and Avi-Tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.

Expression System

CHO

Species

Human

Tag

C-6×His and Avi

Predicted N Terminal

Met17

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 29 kDa
SDS-PAGE: 45-52 kDa, under reducing conditions

Protein length

Met17-His259

Bio-activity

Measured by its binding ability in a functional ELISA. When Human Siglec-3/CD33 (6C5/2) Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag binds with an ED50 of 60-360 pg/mL. Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Subramanian, S. et al. (2006) Blood 107:2548. The ED50 for this effect is 0.05-0.4 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Biotinylated via Avi-tag.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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