Recombinant Human CD33 Protein (Met17-His259), C-6×His and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10602
Official Symbol
CD33
Product Overview
Biotinylated recombinant human Siglec-3/CD33 protein (Met17-His259) with a 6-His-Tag and Avi-Tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
CHO
Species
Human
Tag
C-6×His and Avi
Predicted N Terminal
Met17
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 29 kDa
SDS-PAGE: 45-52 kDa, under reducing conditions
Protein length
Met17-His259
Bio-activity
Measured by its binding ability in a functional ELISA. When Human Siglec-3/CD33 (6C5/2) Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag binds with an ED50 of 60-360 pg/mL. Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Subramanian, S. et al. (2006) Blood 107:2548. The ED50 for this effect is 0.05-0.4 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Human Siglec-3/CD33 (6C5/2) Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag binds with an ED50 of 60-360 pg/mL. Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Subramanian, S. et al. (2006) Blood 107:2548. The ED50 for this effect is 0.05-0.4 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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