Recombinant Human CD33 Protein (Met17-His259), C-6×His-tagged
Product Description
Cat
IMP-10390
Official Symbol
CD33
Product Overview
Recombinant human Siglec-3/CD33 protein (Met17-His259) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Met17
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 28 kDa
SDS-PAGE: 44-49 kDa, under reducing conditions
Protein length
Met17-His259
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.1-0.8 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.1-0.8 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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