Recombinant Human CD34 Protein (32-290aa), N-His-Tagged
Product Description
Cat
IMP-9344
Official Symbol
CD34
Product Overview
Recombinant human CD34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description
Hematopoietic progenitor cell antigen CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is likely an adhesion molecule with a role in early hematopoiesis by mediating attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.
Expression System
E. coli
Species
Human
Tag
N-His
Form
Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT
Molecular Mass
29.7 kDa (280 aa) confirmed by MALDI-TOF
Protein length
32-290
AA Sequence
MGSSHHHHHHSSGLVPRGSHMSLDNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYSQKT
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.25 mg/mL (determined by Bradford assay)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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