Recombinant Human CD38 protein, C-mFc tag

Product Description

Cat

IMP-677

Official Symbol

CD38

Product Overview

Recombinant Human CD38(P28907)(Val43-Ile300) protein, fused with a mFc tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-mFc tag

Form

Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0.

Molecular Mass

The protein has a calculated MW of 56.5kDa, and migrates as 70-90kDa under reducing conditions.

Protein length

Val43-Ile300

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Anti-Human CD38 mAb at 5μg/ml (100 μl/well) can bind Human CD38-mFc. The ED50 of Human CD38-mFc is 17.84 ng/ml.

Bioactivity-BLI 1

Loaded Human CD38-mFc on AMC Biosensor, can bind Anti-Human CD38 mAb-Fc with an affinity constant of 0.54 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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