Recombinant Human CD38 Protein, N-His-Avi tag, Biotinylated

Product Description

Cat

IMP-961

Official Symbol

CD38

Product Overview

Biotinylated Recombinant Human CD38(P28907)(Val43-Ile300) Protein, fused with a 6*His, Avi tag at the N-terminus, is expressed from HEK293 cells.

Expression System

HEK293

Species

Human

Tag

N-His-Avi tag

Form

Lyophilized from sterile PBS, pH 7.4.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 33.3kDa, and migrates as 40-50kDa under reducing conditions.

Protein length

Val43-Ile300

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Notes

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Anti-Human CD38 mAb at 2μg/ml (100 μl/well) can bind Human CD38-His-Avi. The ED50 of Human CD38-His-Avi is 24.54 ng/ml.

Bioactivity-BLI 1

Loaded Biotinylated Human CD38-His on HIS1K Biosensor, can bind Anti-Human CD38 mAb-Fc with an affinity constant of 0.006 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry