Recombinant Human CD3D Protein (Phe22-Ala105), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10387
Official Symbol
CD3D
Product Overview
Biotinylated recombinant human CD3 delta/CD3d protein (Phe22-Ala105) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Phe22
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 38 kDa
SDS-PAGE: 54-61 kDa, under reducing conditions
Protein length
Phe22-Ala105
Bio-activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 epsilon Fc Chimera Protein is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 epsilon Fc Chimera Protein is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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