Recombinant Human CD3D&CD3E Protein, C-Flag&C-His-tagged, Biotinylated
Product Description
Cat
IMP-4230
Official Symbol
CD3D&CD3E
Product Overview
Recombinant extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. The purified protein was biotinylated in vitro.
Description
The CD3 complex mediates signal transduction.
Expression System
HEK293
Species
Human
Tag
C-Flag&C-His
Predicted N Terminal
Phe 22&Asp 23
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human CD3D&CD3E consists of 271 (126+145) amino acids and predicts a molecular mass of 31.05 (14.4+16.65)KDa.
Protein length
Met1-Ala105 and Met1-Asp126
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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