Recombinant Human CD3D&CD3E Protein, C-Flag&C-His-tagged

Product Description

Cat

IMP-4231

Official Symbol

CD3D&CD3E

Product Overview

Recombinant extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.

Description

The CD3 complex mediates signal transduction.

Expression System

HEK293

Species

Human

Tag

C-Flag&C-His

Predicted N Terminal

Phe 22&Asp 23

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of human CD3D&CD3E comprises 271 (126+145) amino acids and has a calculated molecular mass of 31.05 (14.4+16.65)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.

Protein length

Met1-Ala105 and Met1-Asp126

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISAImmobilized human CD3D&CD3E Heterodimer Protein at 2 μg/mL (100 μL/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 7-28 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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