Recombinant Human CD3D&CD3E Protein, No Tag
Product Description
Cat
IMP-4232
Official Symbol
CD3D&CD3E
Product Overview
Recombinant extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Asp126) of human CD3E (P07766-1) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
Description
The CD3 complex mediates signal transduction.
Expression System
HEK293
Species
Human
Tag
No Tag
Predicted N Terminal
Phe 22&Asp 23
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of human CD3D/CD3E consists of 687 (334+353) amino acids and predicts a molecular mass of 77.5 (37.7+39.8) kDa. It migrates as an approximately 44-54 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
Protein length
Met1-Ala105 and Met1-Asp126
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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