Recombinant Human CD3E protein, C-His tag

Product Description

Cat

IMP-1045

Official Symbol

CD3E

Product Overview

Recombinant Human CD3E(P07766)(Asp23-Asp126) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 12.79kDa, and migrates as 18kDa under reducing conditions.

Protein length

Asp23-Asp126

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human CD3E-His at 10 μg/ml (100 μl/well) can bind Human Anti-CD3. The ED50 of Human Anti-CD3 is 3-15 ng/ml.

Bioactivity-BLI 1

Loaded Anti-Human/Monkey CD3E mAb-Fc on Protein A Biosensor, can bind Human CD3E-His with an affinity constant of 1.01 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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