Recombinant Human CD3E protein, C-His tag

Product Description

Cat

IMP-1045

Official Symbol

CD3E

Product Overview

Recombinant Human CD3E(P07766)(Asp23-Asp126) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 12.79kDa, and migrates as 18kDa under reducing conditions.

Protein length

Asp23-Asp126

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human CD3E-His at 10 μg/ml (100 μl/well) can bind Human Anti-CD3. The ED50 of Human Anti-CD3 is 3-15 ng/ml.

Bioactivity-BLI 1

Loaded Anti-Human/Monkey CD3E mAb-Fc on Protein A Biosensor, can bind Human CD3E-His with an affinity constant of 1.01 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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