Recombinant Human CD3E protein, C-mFc Tag
Product Description
Cat
IMP-517
Official Symbol
CD3E
Product Overview
Recombinant Human CD3E(NP_000724.1)(Gln22-Thr48) is expressed in HEK293, fused with a mFc tag at the C-terminus.
Description
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Expression System
HEK293
Species
Human
Tag
C-mFc Tag
Form
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Molecular Mass
32-40kDa, reducing conditions.
Protein length
Gln22-Thr48
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human CD3E-mFc at 10μg/ml (100 μl/well) can bind Human Anti-CD3. The ED50 of Human CD3E-mFc is 9.4 ng/ml.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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