Recombinant Human CD3E protein, C-mFc Tag
Product Description
Cat
IMP-517
Official Symbol
CD3E
Product Overview
Recombinant Human CD3E(NP_000724.1)(Gln22-Thr48) is expressed in HEK293, fused with a mFc tag at the C-terminus.
Description
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Expression System
HEK293
Species
Human
Tag
C-mFc Tag
Form
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Molecular Mass
32-40kDa, reducing conditions.
Protein length
Gln22-Thr48
Endotoxin
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human CD3E-mFc at 10μg/ml (100 μl/well) can bind Human Anti-CD3. The ED50 of Human CD3E-mFc is 9.4 ng/ml.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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