Recombinant Human CD3E&CD3G Protein, C-hFc-Flag&C-mFc-tagged
Product Description
Cat
IMP-4239
Official Symbol
CD3E&CD3G
Product Overview
Recombinant extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal FLAG-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified.
Description
The CD3 complex mediates signal transduction.
Expression System
HEK293
Species
Human
Tag
C-hFc-Flag&C-mFc
Predicted N Terminal
Asp 23&Gln 23
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of human CD3E/CD3G comprises 681 (353+328) amino acids and has a calculated molecular mass of 76.9 (39.8+37.1) KDa. As a result of glycosylation, the apparent molecular mass of human CD3E/CD3G heterodimer is approximately 42-47 KDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Asp126 and Met1-Ser116
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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