Recombinant Human CD3E&CD3G Protein, C-lFc-tagged

Product Description

Cat

IMP-4240

Official Symbol

CD3E&CD3G

Product Overview

Recombinant human CD3E (NP_000724.1) (Met1-Asp126) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 1; Recombinant human CD3G (NP_000064.1) (Met1-Ser113) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified.

Description

The CD3 complex mediates signal transduction.

Expression System

HEK293

Species

Human

Tag

C-lFc

Predicted N Terminal

Asp 23&Gln 23

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human CD3E/CD3G consists of 696 (353+343) amino acids and has a calculated molecular mass of 78.4 (39.7+38.7) KDa.

Protein length

Met1-Asp126 and Met1-Ser113

Endotoxin

< 0.01 EU/μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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