Recombinant Human CD3G Protein (Gln23-Ser116), C-hFc-tagged
Product Description
Cat
IMP-10385
Official Symbol
CD3G
Product Overview
Recombinant human CD3 gamma Fc Chimera protein (Gln23-Ser116) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.
Expression System
HEK293
Species
Human
Tag
C-hFc
Predicted N Terminal
Gln23 inferred from enzymatic pyroglutamate treatment revealing Ser24
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 37 kDa
SDS-PAGE: 42-53 kDa, under reducing conditions.
Protein length
Gln23-Ser116
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 gamma Fc Chimera is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-4 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 gamma Fc Chimera is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-4 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
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  • 1-631-559-9269
    1-516-512-3133
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