Human CD40 Protein, Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD40 Protein, Fc Tag (MALS verified) was more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Immobilized Human CD40 Ligand, His,Flag Tag (active trimer) at 5 ug/mL (100 uL/well) can bind Human CD40, Fc Tag with a linear range of 0.02-0.156 ug/mL.
Loaded Human CD40, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) with an affinity constant of 1.58 nM as determined in BLI assay (ForteBio Octet Red96e).
FACS analysis shows that Human CD40, Fc Tag (HPLC-verified) can bind to 293T cells overexpressing human CD40L. The concentration of Human CD40 is 0.1 ug/mL.