Recombinant Human CD40 protein, C-mFc Tag

Product Description

Cat

IMP-156

Official Symbol

CD40

Product Overview

Recombinant Human CD40 protein(AA Glu 21-Arg 193)(Accession # P25942-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 46.1 kDa. The protein migrates as 58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 21-Arg 193

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) was more than 90% and the molecular weight of this protein is around 110-125 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human CD40 Ligand, Fc Tag at 5 ug/mL (100 uL/well) can bind Human CD40, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 20-156 ng/mL.

Bioactivity-BLI 1

Loaded Human CD40, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) with an affinity constant of 0.911 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS analysis shows that Human CD40, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cells overexpressing human CD40L. The concentration of Human CD40 is 0.03 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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