Recombinant Human CD40 Protein (Glu21-Arg193), C-hFc and 6×His-tagged
Product Description
Cat
IMP-10357
Official Symbol
CD40
Product Overview
Recombinant human CD40/TNFRSF5 protein (Glu21-Arg193) with a Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc and 6×His
Predicted N Terminal
Glu21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 47 kDa
SDS-PAGE: 58-66 kDa, reducing conditions
Protein length
Glu21-Arg193
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD40/TNFRSF5 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 that produces 50% optimal binding is 4-24 ng/mL. Measured by its ability to inhibit rhCD40 Ligand-induced IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD40. The ED50 for this effect is 0.08-0.8 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD40/TNFRSF5 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 that produces 50% optimal binding is 4-24 ng/mL. Measured by its ability to inhibit rhCD40 Ligand-induced IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD40. The ED50 for this effect is 0.08-0.8 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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