Recombinant Human CD40 Protein (Glu21-Arg193), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10369
Official Symbol
CD40
Product Overview
Biotinylated recombinant human CD40/TNFRSF5 protein (Glu21-Arg193) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Glu21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 48 kDa
SDS-PAGE: 61-66 kDa, under reducing conditions
Protein length
Glu21-Arg193
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD40 Ligand/TNFSF5 Fc (HEK293-expressed, mobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag that produces 50% optimal binding is 40-320 ng/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD40 Ligand/TNFSF5 Fc (HEK293-expressed, mobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag that produces 50% optimal binding is 40-320 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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