Recombinant Human CD40LG Protein (Glu108-Leu261), Animal-Free
Product Description
Cat
IMP-10366
Official Symbol
CD40LG
Product Overview
Recombinant human CD40 Ligand/TNFSF5 (Glu108-Leu261) with an N-terminal Met was expressed in E. coli and produced using non-animal reagents in an animal-free laboratory.
Description
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Expression System
E. coli
Species
Human
Tag
Tag Free
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.
Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. The ED50 for this effect is 1-3 μg/mL in the presence of 20 ng/mL of recombinant human IL·4.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, 2 to 8 centigrade under sterile conditions after reconstitution.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
