Recombinant Human CD40LG Protein (Glu108-Leu261), Animal-Free
Product Description
Cat
IMP-10366
Official Symbol
CD40LG
Product Overview
Recombinant human CD40 Ligand/TNFSF5 (Glu108-Leu261) with an N-terminal Met was expressed in E. coli and produced using non-animal reagents in an animal-free laboratory.
Description
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Expression System
E. coli
Species
Human
Tag
Tag Free
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.
Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. The ED50 for this effect is 1-3 μg/mL in the presence of 20 ng/mL of recombinant human IL·4.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, 2 to 8 centigrade under sterile conditions after reconstitution.
