Recombinant Human CD40LG Protein (Glu108-Leu261)

Product Description

Cat

IMP-10367

Official Symbol

CD40LG

Product Overview

Recombinant human CD40 Ligand/TNFSF5 protein (Glu108-Leu261) with an N-terminal Met was expressed in E. coli.

Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

Expression System

E. coli

Species

Human

Tag

Tag Free

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 16.9 kDa (monomer)
SDS-PAGE: 18 kDa, reducing conditions

Protein length

Glu108-Leu261

Bio-activity

Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. The ED50 for this effect is 1-3 μg/mL in the presence of 20 ng/mL of recombinant human IL-4.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, 2 to 8 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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