Recombinant Human CD40LG Protein (Met113-Leu261), N-HA-tagged

Product Description

Cat

IMP-10364

Official Symbol

CD40LG

Product Overview

Recombinant human CD40 Ligand/TNFSF5 protein (Met113-Leu261) with YPYDVPDYA HA tag at N-terminus was expressed in Human embryonic kidney cell.

Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

Expression System

HEK293

Species

Human

Tag

N-HA

Predicted N Terminal

Tyr (of HA tag)

Form

Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.

Molecular Mass

Predicted Molecular Mass: 22 kDa
SDS-PAGE: 20-27 kDa, under reducing conditions.

Protein length

Met113-Leu261

Bio-activity

Measured in a cell proliferation assay using human B cells. The ED50 for this effect is 0.250-3.00 ng/mL in the presence of Recombinant Human IL-4 and a cross linking HA Tag Antibody.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured in a cell proliferation assay using human B cells. The ED50 for this effect is 0.250-3.00 ng/mL in the presence of Recombinant Human IL-4 and a cross linking HA Tag Antibody.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry