Recombinant Human CD40LG protein, N-hFc Tag

Product Description

Cat

IMP-2502

Official Symbol

CD40LG

Product Overview

Recombinant Human CD40LG protein(113-261aa), fused with N-hFc Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-hFc Tag

Form

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.

Molecular Mass

The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD40 Ligand is approximately 40-53 kDa due to glycosylation.

Protein length

113-261aa

Purity

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Biological Activity

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40 Ligand,hFc tagged protein can bind Human CD40, mFc-His tagged protein in a linear range of 0.51-320 ng/ml.

Storage

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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