Recombinant Human CD40LG protein, N-His Tag

Product Description

Cat

IMP-179

Official Symbol

CD40LG

Product Overview

Recombinant Human CD40LG protein(AA Glu 108-Leu 261)(Accession # P29965-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the N-terminus.

Expression System

HEK293

Species

Human

Tag

N-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.0 kDa. The protein migrates as 23 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 108-Leu 261

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD40 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human CD40 Ligand, His Tag at 5 ug/mL (100 uL/well) can bind Human CD40, Fc Tag with a linear range of 0.156-5 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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