Recombinant Human CD40LG Protein, Tag free

Product Description

Cat

IMP-9277

Official Symbol

CD40LG

Product Overview

Human CD40LG (P29965) recombinant protein expressed in E. coli.

Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

Expression System

E. coli

Species

Human

Tag

Tag free

Form

Lyophilized from 10 mM Na2PO4, pH 7.5

Molecular Mass

16.3 kDa

AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Endotoxin

< 0.1 EU/μg

Applications

Functional Study; SDS-PAGE

Storage

Store at -20 centigrade on dry atmosphere. After reconstitution with sterilized water, store at -20 centigrade or lower. Aliquot to avoid repeated freezing and thawing.

SDS-PAGE

1 μg/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue Lane 1: non-reducing conditions Lane 2: reducing conditions

Bioactivity-ELISA 1

Serial dilutions of human CD40LG, starting at 2000 ng/mL, were added to human PBMCs. After 48 hours, cell supernatant was collected and human IL-8 was measured via ELISA and the linear portion of the curve was us used to calculate the ED50.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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