Recombinant Human CD44 Protein (Gln21-Thr222, NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHST, Arg223-Trp269), C-6×His-tagged
Product Description
Cat
IMP-10359
Official Symbol
CD44
Product Overview
Recombinant human CD44v5 protein (Gln21-Thr222, NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHST, Arg223-Trp269) with a 6×His tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Gln21
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 32 kDa
SDS-PAGE: 76-86 kDa, under reducing conditions.
Protein length
Gln21-Thr222, NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHST, Arg223-Trp269
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD44v5 His-tag Protein is immobilized at 2.00 μg/mL (100 μL/well), Recombinant Human LSECtin/CLEC4G binds with an ED50 of 5.00-30.0 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD44v5 His-tag Protein is immobilized at 2.00 μg/mL (100 μL/well), Recombinant Human LSECtin/CLEC4G binds with an ED50 of 5.00-30.0 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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