Recombinant Human CD44 Protein (Gln21-Thr222, NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTST, Arg223-Trp269), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10362
Official Symbol
CD44
Product Overview
Biotinylated recombinant human CD44v5 protein (Gln21-Thr222, NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTST, Arg223-Trp269) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Gln21 inferred from enzymatic pyroglutamate treatment revealing Ile22.
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 60 kDa
SDS-PAGE: 100-120 kDa, under reducing conditions.
Protein length
Gln21-Thr222, NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTST, Arg223-Trp269
Bio-activity
Measured by its binding ability in a functional ELISA. When 2.00 μg/mL of Biotinylated Recombinant Human CD44v5 Fc Chimera Avi-tag protein is immobilized onto Streptavidin coated plate Recombinant Human LSECtin/CLEC4G binds with an ED50 of 10.0-60.0 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When 2.00 μg/mL of Biotinylated Recombinant Human CD44v5 Fc Chimera Avi-tag protein is immobilized onto Streptavidin coated plate Recombinant Human LSECtin/CLEC4G binds with an ED50 of 10.0-60.0 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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