Recombinant Human CD46 Protein (Cys35-Asp328), C-10×His-tagged
Product Description
Cat
IMP-10347
Official Symbol
CD46
Product Overview
Recombinant human CD46 protein (Cys35-Asp328) with a C-terminal 10-His tag was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
Expression System
HEK293
Species
Human
Tag
C-10×His
Predicted N Terminal
Cys35
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 34 kDa
SDS-PAGE: 40-67 kDa, under reducing conditions
Protein length
Cys35-Asp328
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD46 is immobilized at 5 μg/mL (100 μL/well), the concentration Human C4b that produces 50% of the optimal binding response is 2-12 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD46 is immobilized at 5 μg/mL (100 μL/well), the concentration Human C4b that produces 50% of the optimal binding response is 2-12 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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