Recombinant Human CD47 protein, C-Fc Tag

Product Description

Cat

IMP-257

Official Symbol

CD47

Product Overview

Recombinant Human CD47 protein(AA Gln 19-Pro 139)(Accession # NP_942088) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 19-Pro 139

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human CD47, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HPLC

The purity of Human CD47, Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Human CD47, Fc Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Human SIRP alpha, His Tag (HPLC-verified) with a linear range of 0.016-0.5 ug/mL.

Bioactivity-ELISA 2

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (HPLC-verified): Biotinylated Human SIRP alpha, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 ug/mL.

Bioactivity-BLI 1

Immobilized Human CD47, Fc Tag (HPLC-verified) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) with an affinity constant of 0.72 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that Human CD47, Fc Tag (HPLC-verified) can bind to ACHN cell expressing SIRP-a. The concentration of CD47 used is 10 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 ug/mL. IC50=15.02 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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