Recombinant Human CD47 protein, C-His-Avi Tag

Product Description

Cat

IMP-183

Official Symbol

CD47

Product Overview

Unconjugated Recombinant Human CD47 protein(AA Gln 19-Pro 139)(Accession # Q08722-3) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag

Form

Lyophilized from sterile 10 mM Bicine, pH8.3, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 16.9 kDa. The protein migrates as 33-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 19-Pro 139

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Unconjugated Human CD47, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human SIRP alpha, Fc Tag at 2 ug/mL (100 uL/well) can bind Unconjugated Human CD47, His,Avitag with a linear range of 2-13 ng/mL.

Bioactivity-ELISA 2

Immobilized Anti-CD47 MAb, Human IgG4 at 2 ug/mL (100 uL/well) can bind Unconjugated Human CD47, His,Avitag with a linear range of 0.05-2 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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