Recombinant Human CD47 protein, C-His-Avi Tag
Product Description
Cat
IMP-183
Official Symbol
CD47
Product Overview
Unconjugated Recombinant Human CD47 protein(AA Gln 19-Pro 139)(Accession # Q08722-3) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag
Form
Lyophilized from sterile 10 mM Bicine, pH8.3, 10% trehalose.
Molecular Mass
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 16.9 kDa. The protein migrates as 33-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Gln 19-Pro 139
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Unconjugated Human CD47, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human SIRP alpha, Fc Tag at 2 ug/mL (100 uL/well) can bind Unconjugated Human CD47, His,Avitag with a linear range of 2-13 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Anti-CD47 MAb, Human IgG4 at 2 ug/mL (100 uL/well) can bind Unconjugated Human CD47, His,Avitag with a linear range of 0.05-2 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
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