Recombinant Human CD47 protein, C-His Tag

Product Description

Cat

IMP-258

Official Symbol

CD47

Product Overview

Recombinant Human CD47 protein(AA Gln 19-Pro 139)(Accession # NP_942088) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.6 kDa. The protein migrates as 33-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 19-Pro 139

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Human CD47, His Tag with a linear range of 6-50 ng/mL.

Bioactivity-ELISA 2

Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 ug/mL (100 uL/well) can bind Human CD47, His Tag with a linear range of 0.4-3 ng/mL.

Bioactivity-BLI 1

Loaded Anti-Human CD47 MAb (Human IgG4) on AHC Biosensor, can bind Human CD47, His Tag with an affinity constant of 6.18 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human CD47, His Tag with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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