Recombinant Human CD47 protein, C-His Tag

Product Description

Cat

IMP-258

Official Symbol

CD47

Product Overview

Recombinant Human CD47 protein(AA Gln 19-Pro 139)(Accession # NP_942088) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.6 kDa. The protein migrates as 33-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 19-Pro 139

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Human CD47, His Tag with a linear range of 6-50 ng/mL.

Bioactivity-ELISA 2

Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 ug/mL (100 uL/well) can bind Human CD47, His Tag with a linear range of 0.4-3 ng/mL.

Bioactivity-BLI 1

Loaded Anti-Human CD47 MAb (Human IgG4) on AHC Biosensor, can bind Human CD47, His Tag with an affinity constant of 6.18 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human CD47, His Tag with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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