Human CD47, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human SIRP alpha, His Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Human CD47, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 1-31 ng/mL.
Loaded Human CD47, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) with an affinity constant of 1.1 uM as determined in BLI assay (ForteBio Octet Red96e).
FACS assay shows that Human CD47, Mouse IgG2a Fc Tag, low endotoxin can bind to ACHN cell expressing human SIRP-a. The concentration of CD47 used is 10 ug/mL.
FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 ug/mL. IC50=8.39 ug/mL