Recombinant Human CD47 Protein (Gln19-Pro139), C-mFc-tagged
Product Description
Cat
IMP-10337
Official Symbol
CD47
Product Overview
Recombinant human CD47 protein (Gln19-Pro139) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-mFc
Predicted N Terminal
Gln19
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 41 kDa
SDS-PAGE: 58-68 kDa, under reducing conditions.
Protein length
Gln19-Pro139
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP alpha/CD172a Fc Chimera is immobilized at 0.2 μg/mL (100 μL/well), Recombinant Human CD47 Mouse IgG2a Fc Chimera binds with an ED50 of 30.0-300 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP alpha/CD172a Fc Chimera is immobilized at 0.2 μg/mL (100 μL/well), Recombinant Human CD47 Mouse IgG2a Fc Chimera binds with an ED50 of 30.0-300 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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