Recombinant Human CD48 Protein (27-220aa), C-His-Tagged
Product Description
Cat
IMP-9452
Official Symbol
CD48
Product Overview
Recombinant human CD48, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
CD48, also known as CD48 antigen isoform 1, is a GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules. This protein is competent to transduce signals and can also trigger signaling through CD2 or 2B4. CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells. High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation. It expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation.
Expression System
Baculovirus
Species
Human
Tag
C-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
23.4 kDa (203 aa)
Protein length
27-220
AA Sequence
<ADP>QGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKYFESKFKGRVRLDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIKLQVLDPVPKPVIKIEKIEDMDDNCYLKLSCVIPGESVNYTWYGDKRPFPKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKNGTVCLSPPCTLARS<HHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Human CD48/SLAMF2 is coated at 2 μg/mL (100 μL/well) can bind Human CD244 in a Functional ELISA assay.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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