Recombinant Human CD48 Protein, C-His&AVI-tagged, Biotinylated
Product Description
Cat
IMP-1341
Official Symbol
CD48
Product Overview
Recombinant human CD48 (NP_001769.2)(Met1-Ser220) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Description
Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Expression System
HEK293
Species
Human
Tag
C-His&AVI
Predicted N Terminal
Gln 27
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human CD48 consists of 447 amino acids and predicts a molecular mass of 50.88 kDa. It migrates as an approximately 63.75 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Ser220
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human 2B4/CD244 (His Tag) at 2 μg/mL (100 μL/well) can bind Human CD48 (hFc&AVI Tag), Biotinylated, the EC50 of Human CD48 (hFc&AVI Tag), Biotinylated is 15-120 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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