Recombinant Human CD48 Protein, C-His&AVI-tagged, Biotinylated

Product Description

Cat

IMP-1341

Official Symbol

CD48

Product Overview

Recombinant human CD48 (NP_001769.2)(Met1-Ser220) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Description

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Expression System

HEK293

Species

Human

Tag

C-His&AVI

Predicted N Terminal

Gln 27

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human CD48 consists of 447 amino acids and predicts a molecular mass of 50.88 kDa. It migrates as an approximately 63.75 kDa band in SDS-PAGE under reducing conditions.

Protein length

Met1-Ser220

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-ELISA 1

Immobilized Human 2B4/CD244 (His Tag) at 2 μg/mL (100 μL/well) can bind Human CD48 (hFc&AVI Tag), Biotinylated, the EC50 of Human CD48 (hFc&AVI Tag), Biotinylated is 15-120 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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