Recombinant Human CD48 Protein, C-His&AVI-tagged, Biotinylated
Product Description
Cat
IMP-1342
Official Symbol
CD48
Product Overview
Recombinant human CD48 (NP_001769.2) (Met1-Ser220) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Description
Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Expression System
HEK293
Species
Human
Tag
C-His&AVI
Predicted N Terminal
Gln27
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human CD48 consists of 220 amino acids and predicts a molecular mass of 25.60 kDa. It migrates as an approximately 42.34 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Ser220
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human 2B4/CD244 (hFc Tag) at 2 μg/mL (100 μL/well) can bind Human CD48 (His&AVI Tag), Biotinylated, the EC50 is 120-520 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info