Recombinant Human CD52 Protein (Gly25-Ser36), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10342

Official Symbol

CD52

Product Overview

Biotinylated recombinant human CD52 protein (Gly25-Ser36) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

Involved in positive regulation of cytosolic calcium ion concentration. Predicted to be located in extracellular region and plasma membrane. Predicted to be intrinsic component of plasma membrane. Predicted to be active in sperm midpiece.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Ser24&Gly25

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 30 kDa
SDS-PAGE: 38-44 kDa, under reducing conditions.

Protein length

Gly25-Ser36

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human SIGLEC-10 Fc Chimera Protein is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human CD52 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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